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following removal of the 24 amino acid signal peptide the 396 amino acid peptide is cleaved by proprotein convertases 1/3 (PC 1/3) and PC2; nesfatin-1 consists of amino acids 1–82; proteolytic processing also generates nesfatin-2 (amino acids 85–163) and nesfatin-3 (amino acids 166–396); stimulates reduced feeding via actions in the hypothalamus Neurotensin enteroendocrine N cells along the small intestine and proximal large intestine; also in hypothalamus a 13-amino acid peptide derived from a precursor that also produces neuromedin; involved in satiety responses and slows gastric emptying; also involved in nociception (sensation of pain) Obestatin primary site is stomach, minor synthesis in intestine derived from pro-ghrelin protein; acts in opposition to ghrelin action on appetite Oxyntomodulin enteroendocrine L cells predominantly in the ileum and colon contains all of the amino acids of glucagon (see Figure below); inhibits meal-stimulated gastric acid secretion similar to GLP-1 and GLP-2 action; 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